| Mile | Delta | Dir. | Description |
| Begin - Gaithersburg Railroad Station | |||
| 0.0 | L | West on Diamond Ave @ Light | |
| 0.5 | 0.50 | L | Chestnut St. |
| 0.7 | 0.20 | X | West Diamond Ave.- Chestnut becomes Muddy Branch Rd.- Sidewalk |
| 3.1 | 2.40 | X | Great Seneca Hwy. |
| 3.7 | 0.60 | X | Rt. 28 - Use path on left then cross to right shoulder. |
| 4.0 | 0.30 | BL | Becomes Dufief Mill Rd.- Shoulder |
| 6.0 | 2.00 | BR | Travilah Rd. @ Elementary School - Shoulder |
| 6.6 | 0.60 | R | Split Creek Ct.- avoids Travilah traffic |
| 6.8 | 0.20 | L | Stone Barn La. |
| 7.4 | 0.60 | L | Squires Ct. |
| 7.6 | 0.20 | L | Glen Rd. |
| 7.9 | 0.30 | R | Travilah Rd. (Shopping Center - Convenience Store)- on road, use caution. |
| 9.3 | 1.40 | R | River Rd. - Shoulder |
| 9.4 | 0.10 | L | Pennyfield Lock Rd. |
| 10.2 | 0.80 | L | At C & O Canal on road to lock, cross canal |
| 10.5 | 0.30 | R | C & O Canal |
| 13.1 | 2.60 | Lock 23 @ Violettes Lock Road | |
| 13.8 | 0.70 | Lock 24 @ Seneca Creek Aquaduct | |
| 22.3 | 8.50 | Lock 25 @ Edwards Ferry Rd. | |
| 27.5 | 5.20 | L | Whites Ferry Rd. (Parking, Restrooms, Food) |
| 27.5 | 0.00 | X | Potomac River (Ferry $1.00) to White's Ferry Rd. |
| 29.2 | 1.70 | L | Rt. 15 - Shoulder - Caution |
| 31.7 | 2.50 | BR | Business Rt. 15 |
| 32.5 | 0.80 | L | W & OD Trail |
| 33.9 | 1.40 | XU | Rt. 15 |
| 36.9 | 3.00 | X | Goose Creek |
| 37.1 | 0.20 | Quarry Overlook | |
| 41.4 | 4.30 | Smith's Switch Station ( Restrooms, Vending, Water, Air) | |
| 43.2 | 1.80 | XO | Rt. 28 (Parking) |
| 46.5 | 3.30 | X | Crestview Dr. (Convenience Store on Left) |
| 47.0 | 0.50 | X | Ferndale Ave. (Herndon Community Center & Golf Course, Food,Restrooms, Water) |
| 49.3 | 2.30 | X | Reston Pkwy. |
| 51.6 | 2.30 | XU | Dulles Toll Rd. |
| 52.0 | 0.40 | X | Hunter Mill Rd. - CAUTION! |
| 56.2 | 4.20 | Vienna Train Station (water) | |
| 59.2 | 3.00 | XO | Capital Beltway |
| 59.7 | 0.50 | XO | I-66 @ Virginia Ave. Follow Ave. to trail |
| 60.5 | 0.80 | X | Shreve Rd. - CAUTION! |
| 61.1 | 0.60 | XO | Leesburg Pike |
| 62.5 | 1.40 | X | Rt. 29 |
| 68.5 | 6.00 | R | Shirlington Rd. (End W&OD) - Sidewalk |
| 68.6 | 0.10 | R | Arlington Mill Dr. - Sidewalk |
| 68.7 | 0.10 | L | Quincy St. - Sidewalk |
| 68.9 | 0.20 | L | 28th St. - Sidewalk |
| 69.0 | 0.10 | L | Pedestrian Bridge - Cross I-395 |
| 69.2 | 0.20 | L | Martha Custis Dr. becomes Valley Drive - Sidewalk |
| 70.7 | 1.50 | L | West Glebe Rd. - Sidewalk |
| 70.9 | 0.20 | X | West Glebe Rd.@ South Glebe Rd.to Anderson Trail (aka 4 Mile Run) follows stream. |
| 71.4 | 0.50 | XU | Rt.1 and Shopping Center |
| 71.6 | 0.20 | L | Up to Mount Vernon Trail |
| 71.7 | 0.10 | R | North on Mount Vernon Trail |
| 74.7 | 3.00 | Gravelly Point, north of National Airport runway (Parking) | |
| 74.7 | 0.00 | X | Parking lot - continue on trail |
| 76.6 | 1.90 | X | Parking @ Roosevelt Island |
| 76.8 | 0.20 | UP | Ramp to Key Bridge on right |
| 77.1 | 0.30 | X | Key Bridge - Sidewalk |
| 77.4 | 0.30 | R | Thru park to stairs/ramp to C& O Canal path |
| 77.5 | 0.10 | L | On towpath |
| 77.6 | 0.10 | R | On 33rd. St. |
| 77.7 | 0.10 | R | K St. under Whitehurst Frwy. |
| 78.0 | 0.30 | S | Capital Crescent Trailhead |
| 80.2 | 2.20 | Fletchers Boathouse (Parking, Restrooms, Food, Water) | |
| 81.1 | 0.90 | XO | C&O Canal at Arizona Ave. |
| 82.3 | 1.20 | XU | MacArthur Blvd.- Dalecarlia Tunnel |
| 84.0 | 1.70 | XO | River Road (Food) |
| 85.8 | 1.80 | R | Bethesda Ave (trail ends) |
| 85.9 | 0.10 | L | Woodmont Ave.(Use left sidewalk- Ave. becomes one way AGAINST YOU) |
| 86.5 | 0.60 | X | Old Georgetown Rd.-(move to right side on Woodmont - two way again) - Sidewalk |
| 87.1 | 0.60 | L | Rockville Pike - Path/Sidewalk on right side |
| 87.8 | 0.70 | X | Cedar Lane |
| 87.8 | 0.00 | R | On Bike Path |
| 88.4 | 0.60 | XU | Capital Beltway |
| 88.5 | 0.10 | L | Beach Drive (or use Rock Creek Trail) |
| 90.7 | 2.20 | X | Knowles Ave. - trail on right side |
| 91.1 | 0.40 | XU | Railroad tunnel |
| 91.3 | 0.20 | Park (Parking, Restrooms) | |
| 92.8 | 1.50 | X | Randolph Rd. |
| 93.2 | 0.40 | Park on right of trail (Water, Restrooms) | |
| 93.3 | 0.10 | L | SECOND bridge to Dewey Rd. |
| 93.4 | 0.10 | R | Gaynor Lane |
| 93.5 | 0.10 | L | Viers Mill Rd.- Shoulder on Right |
| 94.5 | 1.00 | R | Aspen Hill Rd. |
| 94.5 | 0.00 | L | Adrian St. |
| 94.6 | 0.10 | R | Baltic Ave. |
| 94.7 | 0.10 | L | Parking lot to Rock Creek Trail (Restrooms, Water) |
| 96.6 | 1.90 | XU | Rt. 28 |
| 96.6 | 0.00 | R | Up path (steep!) |
| 96.8 | 0.20 | R | Rt. 28 on shoulder |
| 97.9 | 1.10 | R | East Gude Drive - sidewalks/path on left side |
| 99.8 | 1.90 | R | Crabbs Branch Way - right sidewalk |
| 101.0 | 1.20 | X | Redland Rd. |
| 101.7 | 0.70 | L | Shady Grove Rd. - USE Sidewalk - Caution |
| 102.1 | 0.40 | R | Oakmont Ave. - Caution |
| 102.7 | 0.60 | R | Cross railroad tracks |
| 102.8 | 0.10 | L | Railroad Street |
| 103.1 | 0.30 | L | West on Diamond Ave.- Sidewalk |
| 104.0 | 0.90 | L | Summit Ave. (Parking, Food, Restrooms at train station) |